Page 52 - BJS vol. 35

44 Bangladesh J. Sugarcane, 35 : 37-47 June, 2014

Table 3. Microsatellite primers with corresponding average number of Alleles per
locus, average number of alleles per polymorphic locus, average number
of genotypes per locus together with Polymorphism Information Content
(PIC)

Average Average number Average PIC

Primer number of of alleles per number of (Polymorphism Mean
codes alleles per polymorphic genotypes per Information PIC
locus locus locus Content)
SMC687CS 4.75 19.00 4.75 0.85
SMC334BS 1.64 2.55 1.64 0.90 0.87
SMC119CG 2.17 8.67 2.17 0.88
SMC766BS 4.85 17.00 4.85 0.88
Table 4. Summary of linkage distances for different pairs of sugarcane germplasm

K84- K88- PS81-
Germplasm K76-4 K8887 K8892 PS851 PS862 PS863 PS86-
200 65 362
10029
K84-200 0.0 7.0 12.0 10.0 10.0 12.0 12.0 14.0 10.0 11.0
K76-4 7.0 0.0 11.0 11.0 11.0 13.0 11.0 13.0 13.0 14.0
K88-65 12.0 11.0 0.0 10.0 12.0 14.0 16.0 16.0 14.0 13.0
K8887 10.0 11.0 10.0 0.0 4.0 8.0 12.0 14.0 14.0 11.0
K8892 10.0 11.0 12.0 4.0 0.0 8.0 12.0 16.0 14.0 13.0
PS851 12.0 13.0 14.0 8.0 8.0 0.0 10.0 8.0 10.0 9.0
PS862 12.0 11.0 16.0 12.0 12.0 10.0 0.0 12.0 10.0 11.0
PS863 14.0 13.0 16.0 14.0 16.0 8.0 12.0 0.0 10.0 11.0
PS86-10029 10.0 13.0 14.0 14.0 14.0 10.0 10.0 10.0 0.0 7.0
PS81-362 11.0 14.0 13.0 11.0 13.0 9.0 11.0 11.0 7.0 0.0

M 1 2 3 4 5 6 7 8 9 10
124bp

104bp

89bp

80bp
Figure 1. DNA Fingerprinting of BSRI ten germplasm of sugarcane based on SSR primer
pair SMC119CG through PAGE (M = marker pBR322Haelll, Lane 1 = K84-200,
Lane 2 = K76-4, Lane 3 = K88-65, Lane 4 = K8887, Lane 5 = K8892, Lane 6 =
PS851, Lane 7 = PS862, Lane 8= PS863, Lane 9 = PS86¬-10029 and Lane 10
= PS81-362)