Page 54 - Розбенгалт эсрэгтөрөгчийн чанарын хяналт, розбенгал урвалын үнэлгээ

“Розбенгалт эсрэгтөрөгчийн чанарын хяналт, розбенгал урвалын үнэлгээ” 2017 он

QUALITY CONTROL OF ROSEBENGAL ANTIGEN AND

VALIDATION OF ROSEBENGAL TEST

Jugderkhorloo UNENBAT
Superviser: Vanaabaatar BATBAATAR

Economic impact of brucellosis is extensive and ranked among the most
important zoonosis globally due to posing great damage in livestock productivity and

human health. From diagnostic point of view, rose bengal test (RBT) is most common

and effective screening test with low cost and high sensitivity.
Although ‘Biocombinate’ STATE OWNED company of Mongolia have been

producing rose bengal antigen (RBA) since 1978 for the diagnosis of livestock
brucellosis, evaluation and validation of RBT utilising local reference sera and RBA

produced in many countries still have not been done. Thus, we have aimed to
conduct comparative evaluation of diagnostic capacities of RBA produced at

Biocombinate to RBA produced at ID Vet of France using local reference sera. Prior

to this, we have examined cultivation and dissimilation of B. abortus 99 strain used in
the production of RBA in 9 different selective and basal culture medium such as meat

peptone agar, meat peptone broth, Trypcase soya agar, Tryptone soya agar,

Brucella agar, Blood agar, Agar with Thionine, Streptomycin and Penicillin,
respectively. Biochemical characteristics and motility was identified in liquid and

semi-liquid medium and haemolytic feature was checked by selective “Вlood agar
base” medium.

General characteristics of the B. abortus 99 strain are confirmed to be
conserved which suggested in strain passport of Biocombinate SOSSE and World

Organisation for Animal Health (OIE) listed requirements. Furthermore, we have

examined the B. abortus 99 strain by Bruce-Ladder polymerase chain reaction (PCR)
which was recommended by World Animal Health Organization (OIE) and the B.

abortus 99 strain was confirmed to be not underwent for any genetic mutations or
deletions. Along with this, quality control tests were inspected according to Veterinary

Pharmacopeia-2009 on final product of RBA and quality passed RBA was utilized for
our further examination.

ЖҮГДЭРХОРЛООГИЙН ҮНЭНБАТ 53