Page 55 - Тахианаас хүнсний хордлого үүсгэгч CAMPYLOBACTER SPP.-ийг илрүүлсэн судалгааны дүн

[ШИНЖЛЭХ УХААНЫ МАГИСТРЫН ЗЭРЭГ ГОРИЛСОН БҮТЭЭЛ] Хураангуй
Дүгнэлт

CONCLUSION
1. Multi-plex PCR that distinguishes Campylobacter species revealed that 29 of 36 isolates
were C. jejuni and the remaining 7 isolates were C. coli. This indicates that chicken farms
and farmers were significantly contaminated with these pathological agents.

2. Regarding fluoroquinolone resistance, 20 of 36 isolates (55.6%) were resistant to four
fluoroquinolones, nalidixic acid, ofloxacin, norfloxacin, and ciprofloxacin. Antibiotic Resistance
of Campylobacter spp. was determined by E-test and among tested Campylobacter spp. isolates
55% were resistant to antibiotics of Quinolone group and 97% were resistant to tetracycline.
Therefore, it is necessary to carry out further research on the causes of antibiotic resistance.
3. To confirm the fluoroquinolone resistance, we analyzed nucleotide sequences of GyrA
gene of the isolates. All the fluoroquinolone sensitive isolates possessed threonine at codon 86
as expected; however, all the fluoroquinolone resistant isolates possessed amino aicd
substitution from threonine to isoleucine at codon 86 (nucleotide substitution: ACA to ATA).
Samples of Campylobacter spp tested in our study, confirmed to be affected by antibiotic
residue.
4.To characterize the isolate genetically, Multi Locus Sequence Type (MLST) analysis was
carried out. MLST analysis revealed that many of the isolates belonged to Sequence Type (ST)-
828, ST-574, ST-464, ST-49, ST-22, or ST-21, which are observed worldwide. However, 4
samples of C.jejuni, which detected during our study, have a similar nucleotide sequence with
Campylobacter spp., isolated from China. From these findings, we can assume that there is a

new type of species may be distributed in Central Asia.

55