Page 228 - konishipaper

*Received1Ma，.r201(No.1-0165) [DOI:10.1299~bse.6.1601
Copyright(C)201byJSME
ComparisonofLegionelaBiofilmFormations atThreeDiferentTemperaturesinLiquid Flow，inStaticLiquidandonAgarPlate*
TadashiKONISHI*ぺAkiraNISHIZONO***，TetsuYAMASHIRO**料 andMichioKOIDE*****
対 DepartmentofMechanicalEnginering，OitaNationalColegeofTechnology 16Maki，Oita，Japan E-mail:konishi@oita-c.tac.jp
帥 * FacultyofMedicine，OitaUniversity 1Idai-gaolくa，Yufu，Japan *InstituteofTropicalMedicine，NagasakiUniversity 1-12-4Sakamoto，Nagasaki，Japan *FacultyofMedicine，UniversityoftheRyukyus 1Senbaru，Nishihara，Okinawa，Japan
Abstract ThebiofilmformationofLegionelαpneumophilaonglaswasexaminedunder nutrientwaterf10wconditionat370C，40oC，and420C.Thethre-dimensional structures of 8・days biofilm were analyzed using confocal laser scaning microscopy.Itwasrevealedthatthepropertiesofthebiofilmswereinf1uenced significantly by temperature changes. Equivalent outer diameters of the microcolonies were 39μm at 370C，189μm at 400C and 359μm at 420C， respectively.Themostinterestingobservationwasthatthemicrocoloniesshow areasofholowing.Theholownesoremptinesis37%at370C，52%at400Cand 74%at420C，respectively.Theaveragenumberofbacteriapersinglecolonywas c.a.1x105at370C.Also，bacterialengthswerecomparedamongthebiofilmsin liquidf1ow，thoseinstaticliquidandthoseonBCYE-αagarplate.Intheliquid f1ow，theaveragebacterialengthwas4.4μmat370C，2.9μmat400Cand3.5μmat 420C，respectively，whileinthestaticliquidandonagarplatethelengthsincreased withtheriseoftemperature.Ourexperime川n此1tsresultsonthebiof日ilmformationofL.
pr刀1eu.μoηn10philαawil戸pro吋videthebasisforabet抗te白runde白r叫 andi加ngoft由hesesilemodeof lifeofL.pneumophila.
Keywords:Legionela，Biofilm，FlowChanel，TemperatureEfect，Microcolony
1.Inh'oduction
BacteriaofthegenusLegionelaiswidelyfoundinthenaturalenvironment，andcauses Pontiac fever and Legionel la pneumonia. Legionel la are Gram-negative， aerobic， and rod-shaped with one or more polar f1agel la as shown in Fig.1 (1) (2) The number of Legionelasp.asociatedwithpneumoniawasrarelyreportedinJapanuntilApril19， whenanew“LawConcerningthePreventionofInfectiousDiseasesandPatientswith InfectiousDiseases(NewLawonInfectiousDiseases)"wasenacted.Thenumberof