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ThePosibilityofNewCryopreservationTechnique ofPlantCelunderLowerTemperature
TakakoNinagawa1，AkemiEguchi2ラ AkiraNarumi3¥TadashiKonishi4
1GraduateStudent，KanagawaInstituteofTechnology，Kanagawa243-0292Japan 2ToyoEngineeringWorks，Ltd，242-0001Japan 3Dep.tofMechanicalEngineering，KanagawaInstituteofTechnology，Kanagawa243-0292，Japan 4Dep.tofMechanicalEngineering，OitaNationalColegeofTechnology，Oita870心 152，Japan
宇 1arumi(co，me.kanagawa-it.ac.jo
Thedeve10pmentofcryopreservationtechniquehasatractedspecia1atentionfromwhominterestedinfod s h o r t a g e a n d b i o 1 o g i c a 1 p r e s e r v a t i o n . A s b e i n g w e l l - k n o w n ， it is s a i d t h a t t h e d r e a m o f c r y o p r e s e r v a t i o n techniquekepsalivingthingalivewithoutformingicecrysta1underso10wtemperaturethatanybacteria canotbeactive.Thismeansthatsuperco1ingstatemustbekeptundermuch10wertempera加 rethanfrezing poin.tHowever，becausemostofbiomateria1containsa10tofwaterandsuperco1ingisunstab1e，itisextreme1y dificu1ttorearchthedreamofcryopreservationtechniquetobiomateria.lOntheotherhand，itissaidthatcel 1eadstodeathattheOCUlTenceofintracelu1arfrezing.Therefore，thenewtechniquetogive1esdamagetocel isdesired;thatis，thetechniqueistomaintainsupercolingstateundereven10wertemperatureandtomakeice crysta1finer.Thisresearchwasperformedtofindouttheposibi1ityofreductiontechniqueofdamagetocel duetoiceClγsta1anddiscusedfi'omtheviewpointsoftemperatureatthere1easeofsuperco1ingandthe deformationatOCUlTenceofintracel1u1arfi'ezing.P1antwasusedforalivingthing.Microelectricalcurent was10adedtop1anttisue.Figure1showstherelationshipbetwenelectriccurentandthecel1deformationat ocunenceofintracelularfi'ezing.Figure2showstheefectofe1ectriccunentonthedegreofsuperco1ing (=frezingpointofwater-temperatureatthereleaseofsupercoling).Thebothfigurestel1thatthereisthe posibilityofreducingdamagetocel1by10adingmicroelectriccunenttocel.l
Vc=!'C/mhl
.90.95 ct: 5090
C
~O .SS
占
・1セ=lO'C/min
.~乍= !OO'C/mill
∞.1
e
0.75 ・ー----一一一一一一一一一一一一一一一一一一一 且 70 ナーー一一 ー
Elec凶cCU!Tent1ULA)
10 12
Fig.lTherelationshipbetwenelectriccurent andthedeformationofcel
References
0.1 1 10 EI目 tricCUtrentJ(，凶}
Fig.2Theefectofelectriccurenton temperatureatthere1easeofsupercoling
1.Uemura，M.andYoshida，S.，PlantPhysio，.l80，1986，187圃 195. 2.Murai，M.andYoshida，S.，PlantCelPhysio，.l39(1)， 198，97-105.