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!神奈川工科大学大学院工学研究科機械工学専攻 2神奈川工科大学工学部機械工学科 3岩手大学農学部附属寒冷バイオフロンティアセンター 4大分工業高等専門学校機械工学科
蜂川貴子鳴海明乙河村幸男三小西忠司4 TheLoadEfectofElectricCurrentontheLoweringofIntracelularp HinPlantwiththe
低温生物工学会誌 (ClyobiologyandCryotechnology，J Vo.l60，No.2，13"137，2014
冷却に伴う植物の細胞内 pH低下への電流負荷効果
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ProgressofCooling TakakoNINAGAWA¥AkiraNARUMr， YukioKAWAMURA，TadashiKONISm
1GraduateScholofMech正7l1icalEngi・nel・ing，KanagawalnstituteofTechnolog)J， 1030Shimo-Ogino，Atsl/gi，243-0292Japan 2DepaJ・ 抑 制 of脆 chanicalEnginerinιKanagGYI叫 1stituteof毘Ch7仰 g)I，1030Shimo-Ogino，Atsugi，243-0292Japan 3C!yobiofrontierResearchCente7;FacultyofAgriculture，lwateUniversi，yt3-18-8Ueda，Moバoka，020-850JapaJ1 4DepartmentofMechanicalEnginering，OitaNationalColegeofTechnology，16Mald，Oita，870-0152iIψ an eCorespondingauthor，e-mail:narumi@me.kanagawa-it.acj.p)
Cryopreservation technique is very esential to the various fields，such as medical and fod enginering. Consideringtheworldwidefodshortageduetotheexplosiveincreaseofworld population and 企 equently oc cur ring extraordinarγclimate in near future， the development of cryopreservationtechniqueisdesired.Undertheabovebackground，wehavecontinuedtoinvestigate theloadefectofelectriccurentonplantcelinthecolingandfrezingprocesesinordertodiscus theposibilityofnewclyopreservationtechnique.Wehadfoundthatthel.oadofelectricCUl1'・ent influencedontheicecrystalbehaviorandtempera旬 reattheocurenceofintracelularfrezingand reportedabouttheresultspreviously.Inthisresearch，weinvestigatedhowtheloadofelectriccUITent inf1uencedonintracelularpHinthecolingproces，focusingonintracelularpHwhichisoneof indicatorsofcel1activity.HelianthustuberosusL.andAlliumcepaL.wereusedasplantsfortesting. Intracel1ularpHwasmeasuredusingfluorescenceintensityratiomeity.Asaresu，t1 itisc1earthatthe loadofelectricCU1Tentcauses.thereductionintheloweringofintracelularpHinplantwiththe progresofcoling.
研究報告 [Keywords:Plant，Coljng，lntracelularpH，Electric curentloading;植物，冷却，細胞内 pH，電流負 荷]
(ReceivedJu1.31，2014;AceptedSep.16，2014) 緒日
低温保存技術は，食品，医療などさまざまな分野 において重要な役割を果たしてきた.今後，世界人
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