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T.Nin日E日W 口 etロ.1/Cryobiology73ρ01旬 30-39
(C) 80
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(A)10μA
-10μA
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ロNocurent
回 喝.
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0.0 3.2
16.1 19.4
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1.0
12.9 Length[l-1m]
2.6 25.8 29.0
(D)
1.8
O 10 Electriccurent[μA]
Fig.7.Edgesofintracelularicecrystalswithorwithoutelectriccurentloadingat-1oCfmin.(A)Imageoftheedgeofanintracelularicecrystalwith10同.(B)lmageoftheedge ofanintracelularicecrystalwithoutelectriccurentloading.(C)Brightnesdiferencesattheedgesoftheintracelularicecrystalin(A)and(B).(0)Thecharacteristicgrainsizeof intracelularicecrystalwithorwithoutelectriccurentloading.Thecharacteristicgrainsizedecreaseswithanincreaseoftheelectriccurentloadingvalue.Lower-casealphabetic charactersshowthestatisticalysignificantdiferencesbetweentreatmentsbyone-wayANOVAwithTukey-Kramermethod(Pく 0.05).Bars:SE(n=10).
toencompasshalfoftheinsideofthecelwithorwithoutelectric curentloading.Theedgeoftheintracelularicecrystalgrainwith electric cur rent loading (Fig. 7A) ap peared finer than without electriccurentloading(Fig.7B).Fig.7Cshowsthebrightnesdif- ferenceoftheintracelularicecrystaledgebetweentheimage capturedbeforeintracelularfrezingocuredandtheimagein whichintracelularicecrystalshadocupiedahalfoftheinsideofa celwithorwithoutelectriccurentloading.Itisconsideredthat theedgeofintracelularicecrystalsexistedatthelocationswiththe largerbrightnesdiferences.Whenthemicroelectriccurentwas loaded，thepeaksinthebrightnesdiferencesbecameslightly lowerandmorenumerous，andhadnarowerintervalsbetween them.Itissuposedthatthetethattheedgesoftheforming intracelularicecrystalswithelectriccurentloadingbecomefiner thanwithoutthat.Furthermore，thecharacteristicgrainsizesatthe edgeofintracelularicecrystalwithorwithoutelectriccurent
loadingwereexamined(Fig.70).Thecharacteristicgrainsizeswith electriccurentloadingdecreasedsignificantlytoaproximately 85%at1μAandtoaproximately81%at10μA，ascomparedwith noelectriccurentloading.Itwasindicatedthattheedgesofthe intracelularicecrystalgrainswithelectriccurentloadingwere finerthanwithoutelectriccurentloading.
Thegrowingratesofintracelularicecrystalwithorwithout electriccurentloadingwerealsoevaluatedusingtheareavelocity ofthose(Fig.8).Althoughtheareasofintracelularicecrystals imediatelyaftertheirapearanceinsideacelwerethesame acrosalcolingrates，therelationbetweentheareaofintracel- lularice crystals andwith orwithoutelectriccurentloading becamestatisticalysignificantwithincreasedtimesincetheirfirst apearance.Theareasofintracelularicecrystalswithelectric curentloadingat10msecaftertheirfirstapearancedecreasedto aproximately93%at1μAandtoaproximately78%at10μA，as
