Page 5 - EOS Laboratories Price List 2018-20_Neat

TERMS AND CONDITIONS

Prices: Prices quoted in the price list are retail prices applicable at the me of prin ng and are subject to altera on without previous no ce.
Invoice will be made on the basis of prevailing prices at the me of dispatch of the material.
Taxes: Prices quoted are net. 18 % GST Taxes is applicable on all products.
Payment: Payment to be made by cash, by re ring documents through any Scheduled Bank, DD or Duly crossed cheque in favor of EOS
Laboratories Payable at Mumbai. Materials will be dispatched against full payment unless mutually agreed otherwise.
Credit: Credit for payment is at the discre on of management, which cannot be for more than 30 days. Failure to make full payment will
a ract 24 % interest on the invoice value, which will be applicable from the date of Invoice.
Delivery: Dispatches will be made within 30 days a er receiving confirmed order; Packing, forwarding and freight charges will be extra. Orders
for more than Rs. 10,000/‐ will be supplied for by cheapest mode of transport.
Insurance: All Materials are packed with utmost care and are forwarded at customer’s risk. No responsibility is taken for damage or loss in
transit. Goods will be insured on request at 1.5 % of invoice value.
Road Permit: Road permit wherever applicable should be sent along with the order to enable to send the material on me.
Note: No cancella on of orders will be accepted a er the order has been dispatched. Guarantee for quality is subject to storage
condi ons specified on the label of the product. Our products are for diagnos c purpose, in‐vitro use only. No responsibility whatsoever is
taken if products are used for any other purpose.

USERS INSTRUCTION

On receiving the media, check for seal, do not use if seal is broken and inform accordingly. Note down the Batch No, Date for use before and
date of opening the product. Store dehydrated media, ready to use media, an bio c discs and polydiscs as per instruc ons on the label.
Dehydrated culture media are highly hygroscopic. Besides, many media contain some dyes as indicator. Hence, these media should be stored
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in a cool dry place away from light below 25 C.Before opening the bo le, shake and check for lump forma on, lump forma on indicates
deteriora on of medium. Rehydra on: Media are to be recons tuted as per direc on on the label with de‐ionized or dis lled water. Firstly add
the desired quan ty in a por on of water, dissolve by shaking and make up the volume by adding remaining volume of water. If medium
hasn’t dissolved completely, heat directly on the flame or in a water bath ll the medium is dissolved completely.
Common Uses for Media
General Purpose Media‐These support the growth of a large variety of microbes. For example, Nutrient Agar consists of beef extract, peptone
and can be used to culture most of the commonly encountered organisms. Certain other organisms have more complicated requirements for
certain compounds and are called fas dious organisms. These la er organisms must be cultured on media which have been enriched or
supplemented with the par cular growth factors needed by the specific organisms.
Differen al Media‐These media o en contain reagents or chemicals and produce colonies that allow the observer to
differen ate between types of microbes. For example, if a mixture of organisms is cultured on Blood Agar medium, some of the bacterial
colonies may hemolyze the blood cells and produce clear areas in the agar. This permits one to dis nguish between hemoly c and
non‐hemoly c bacteria. pH indicator dyes may also be used to dis nguish between growth producing acidic, neutral or alkaline end products.
Selec ve Media‐These media allow for the growth of only certain types of organisms due to addi on of inhibitors or a specific carbon source.
For example, addi on of Sodium Azide to a medium would inhibit the growth of organisms using respira on but would permit growth of
organisms using only fermenta on. Enrichment culture techniques exploit the principle of survival of the fi est to favor the growth of certain
organisms. These techniques o en involve the use of media that permit one type of organism to outgrow all others. For exampl e, if a
par cular medium contains lactose as the sole fermentable carbohydrate, then the lactose fermen ng organisms present in a mixed culture
would tend to outgrow the other types.
pH Adjustment: Generally dehydrated medium will give desired pH a er recons tu on. However, in case of varia on, adjust the pH with 0.1
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N Hydrochloric acid or 1 N sodium hydroxide. pH of broth and molten medium should be adjusted at 25 C and 40 C ‐50 c respec vely. The pH
value of recons tuted dehydrated culture media should be as men oned on label. It is advisable, however, (par cularly if older material is
being used) to check the pH and correct it if necessary. The pH value depends very much on the composi on of the culture medium, the
temperature at which the pH is measured and the treatment which the culture medium has been subjected to during recons tu on
(dissolving, steriliza on).

Steriliza on: Dispense the recons tuted medium as needed and sterilize by autoclaving as per direc on on the label. Autoclave steriliza on at
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15 pounds pressure (121 C) is recommended for one liter of medium. If larger volumes are to be sterilized in one container and especially if
the medium is not hot, longer period should be employed. Container should o o
Pressure in pounds Temperature( C) Temperature( F)
never be more than two ‐ third full. Autoclave chamber should not be 5 109 228
overloaded to facilitate free flow of steam around the content. Ini ally all the 10 115 240
air in the chamber should be expelled and replaced by the steam to avoid 15 121 250
“hot” and “cold” spots in the chamber. Pressure‐Temperature rela on in an 20 126 29
autoclave with complete replacement of air with steam is shown in the table: 25 130 267
Over steriliza on should be avoided, as it affects the quality of the medium. Problems due to over steriliza on include ‐Darkening of the
medium, destruc on of nutri ve elements, loss of selec ve or differen al proper es, loss of gelling property of agar, change in ph,
decomposi on of carbohydrates, appearance of precipitate and biological/chemical indicators should be used to check the proper func oning
of the autoclave.