Page 6 - EOS Laboratories Price List 2018-20_Neat

Dispensing The Medium: Sterilized PROBLEMS ENCOUNTERED CAUSES
0 0 Lump forma on Prolonged or improper storage
medium should be cooled to 45 ‐50 C
storage un
and poured into petridishes, tubes or Loss of color in indicator medium der direct light
Over steriliza on, prolonged storage,
vials in a laminar flow under asep c Change in pH
condi ons. Leave the lid of petridishes Use of improper ,Water and/or glassware’s
open for 1 ‐2 hours for a dry surface. DEHYDRATED MEDIA Precipita on Over steriliza on
To prepare blood Agar, add 5 ‐10 % Loss of gelling property Overhea ng, improper rehydra on
defibrinated blood a er cooling the Insolubility Insufficient hea ng, improper rehydra on
0
0
medium to 45 ‐50 C and then pour Darkening Overhea ng, improper mixing
into Petri‐dishes. Contamina on Improper steriliza on, not observing asep c
condi ons while pouring, improper container.
Ice forma on Improper Storage
Storage of Ready to Use Media:
Prepared plate media should be stored READY TO USE MEDIA Dried medium Old medium, Improper Storage
0 Loss of color in indicator medium Improper Storage, prolonged exposure to light
at 4 ‐ 10 C in plas c bag or container
to avoid loss of moisture. Media Turbidity in liquid medium Contamina on

contained in tubes with screw cap are Growth in the zone of inhibi on Swarming organisms

preferred than in tubes with co on Loss of potency Improper storage, Old stock

plug. If tubes of media have been kept ANTIBIOTIC DISCS/ POLYDISCS Growth of colonies in the zone of

for long me, they should be reheated inhibi on Mixed culture

before use. Liquid media should be

heated in boiling water bath or in

flowing steam to drive off dissolved atmospheric gases. Agar tubes should be melted and allowed to solidify to get moist surface for good

0
growth of micro‐organisms.Media in petridishes should be dried before use. Do not store media plates below 0 C as it destroys its gel

structure.

0
Storage Of An bio c Discs And Polydiscs: Stock of an bio c discs & polydiscs should be stored at ‐20 C to maintain their potency. Stock for

o
daily use should be stored at 2‐10 C in a refrigerator. Discs should be brought to room temperature before use.

Disposal of used Media: Used culture media may contain highly pathogenic micro‐organisms hence; it should be autoclaved at 15 lbs pressure

for 30 minutes before disposal. Used media in reusable glassware’s should be autoclaved before washing.

Ingredients used in the prepara on of culture media by EOS Laboratories are procured from reputed sources to maintain standard quality.

Strict quality control is maintained by a dedicated team of professionals to ensure proper and reproducible results.

Agar: Agar is used as solidifying agent in culture medium. It gives absolute clarity a er being dissolved and its gelling temperature is around

o
80 C.

Beef Extract: It provides Nitrogen vitamins, amino acids and carbon in culture media.

Bile Salts: Salts and Bile Salt No.3 are selec ve agents incorporated in culture media to provide selec vely for the isola on of gram nega ve

organisms. Bile is derived from liver. Bile Salts No. 3 is a modified frac on of bile acid salts providing a refined bile salt.

Casamino Acids : Casamino acids are obtained from acid hydrolysis of casein which is milk protein and a rich source of nitrogen and growth

factors in culture media.

Carbohydrates: Various carbohydrates are incorporated in culture media, par cularly for use in diagnos c microbiology. It helps in the

iden fica on of micro‐organisms due to their fermenta ve characteris cs of the carbohydrate.

Gela n: It is derived by the hydrolysis of collagen. It is a source of amino acids and nitrogen. It is used in the medium to study gela n

liquefying proper es of the organisms.

Minerals: Various minerals such as Sodium, Potassium, Magnesium, Ferrous or ferric ions in the form of their chlorides, phosphates and

sulphates are used in culture media. Minerals are the essen al requirements for the growth of organisms.

Peptone: Peptone is enzyma c digest of protein. It is rich source of nitrogen in culture media.

Proteose Peptone: It is an enzyma c digest of protein high in proteases. It is incorporated in the culture medium to support the growth of

fas dious micro‐organisms.

Tryptone: Tryptone is a pancrea c digest of casein, a protein of milk. It is rich source of amino acids and nitrogen. Absence of detectable levels

of carbohydrates in tryptone makes it suitable peptone for the fermenta on study of carbohydrates. Tryptone is rich in Tryptophan making it

suitable for detec on of Indole.

Dyes: Different dyes like neutral red, phenol red, Bromothymol blue, brilliant green etc are used in culture media as an indicator of pH or Eh.

Yeast Extract: Yeast extract is incorporated in culture media as an excellent bacterial growth s mulator. It is also source of vitamins, nitrogen,

amino acids and carbon in culture media. Yeast Extract is a water soluble component of autolysed yeast containing vitamin B complex.