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                                                 Answer ALL questions.

                                Write your answers in the spaces provided in this booklet.



            Please begin Question 1 FIRST.


            1.     You are to carry out a simple investigation into the effect of different concentrations of a solution on   DO NOT WRITE IN THIS AREA
                   the tissue of the cucumber fruit.  Read the instructions that follow carefully before beginning.


                           You are provided with the following:

                           •      0.1 M, 0.2 M, 0.3 M, 0.4 M, 0.5 M

                   Remove ten, 2 cm deep sections that are 5 cm long from the cucumber provided, as shown in Figure
                   1 (i) and (ii) below.





















                                  Figure 1. Sections cut from a cucumber                                               DO NOT WRITE IN THIS AREA

                   The strip should have a tough covering of cuticularized epidermis, while the inner part is composed
                   of cortical parenchyma cells.

                   As soon as the strip is cut out of the cucumber, it bends backwards as shown in Figure 2.


















                                         Figure 2                                                                      DO NOT WRITE IN THIS AREA

                    Measure the straight length of the epidermal strips and record the results.
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