Page 160 - SUBSEC October 2017_Neat
P. 160

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             2.     (a)    The rates of enzyme-catalysed reactions can be influenced by the pH at which they occur.
                           Table 1 shows the relative reaction rates for salivary amylase and arginase at different pH
                           values.

                            (i)   On the  graph grid provided, plot a graph of the relative rates for BOTH salivary
                                  amylase and arginase.
                                                                                                   [6 marks]           DO NOT WRITE IN THIS AREA

                                  TABLE 1: REACTION RATES FOR TWO ENZYMES



                                                                     Relative reaction rates
                                        pH Values          Salivary amylase         Arginase (units)
                                                                (units)
                                           4.5                     2.2                        0
                                           5.0                     5.0                        0
                                           5.5                     8.0                      0.2
                                           6.0                   13.0                       1.0
                                           6.5                   17.0                       4.0
                                           7.0                   18.0                       7.0
                                           7.5                   16.0                      9.0

                                           8.0                   11.0                     11.8                         DO NOT WRITE IN THIS AREA
                                           8.5                     6.0                   13.5
                                           9.0                     2.0                   16.0
                                           9.5                                           18.0
                                          10.0                                           17.8
                                          10.5                                           15.0
                                          11.0                                           14.0



                           (ii)   Using the graph, determine the optimal pH for

                                  (a)    arginase activity ...............................................................................................
                                                                                                    [1 mark]

                                  (b)    salivary amylase activity .................................................................................
                                                                                                    [1 mark]           DO NOT WRITE IN THIS AREA














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