Page 11 - Brochure Hibergene
P. 11
Instructions for Use
6.
7.
8.
Centrifuge the sample for 10 secs @ 2700-3300 1 rcf
Add 25μl of lysed samples to target and EC wells, vortex briefly to mix and centrifuge for 10 secs @2700-3300 rcf
Run on HG Swift
8.1.1 Preparation of Enhanced Buffer
Prepare Enhanced Buffer by adding 250μl from an individual COVID-19 Dilution Buffer tube (COVID-DB) to one vial of lyophilised COVID-19 Enhancer (COVID-E) using a calibrated micropipette. Briefly vortex and centrifuge each tube after resuspending. For each sample to be tested, use an individual vial of COVID-19 Enhanced Buffer.
Note: Multiple vials of COVID-19 Enhanced Buffer may be prepared in advance of testing. The reconstituted solution is stable for up to 7 days when stored at 2-8°C.
8.1.2 Sample Collection & Handling
IFU-024 Rev 03 Page 10 of 31
1RCF = Relative Centrifugal Force. This is calculated based on the radius (r) of the rotor (in mm) and the centrifuge
speed in revolutions per minute (rpm) as follows: