Page 12 - Brochure Hibergene
P. 12
Instructions for Use
1. Collectupperrespiratoryswabsampleperapplicableclinicalguidelines. Nasopharyngeal and oropharyngeal swabs may be tested individually or combined in the same transport medium tube. The following transport media are compatible with the test:
3ml VCM transport medium + standard Sigma Swab (Medical Wire Cat. No. MW918S)
3ml Copan UTM + Copan Flocked Swab (Copan, Cat. No. 305C)
3ml Puritan UniTranz + Flocked Swab PurFlock Ultra (Puritan Medical
Products Cat. No. UT-306)
2ml Virocult + mini foam swab (Medical Wire, MW950SENT)
3ml transport medium volumes are recommended where possible. Small swabs suitable for nasopharyngeal sampling should be used.
Swab samples in transport media can be stored at 2-8oC for up to 48 hours prior to testing.
2. Closethecapofthevialcontainingthetransportmediumandswaband vortex for 10 secs. It is recommended to centrifuge briefly to ensure all liquid is at the bottom of the tube.
3. Carefully open the vial containing the transport medium and swab so as not to spill any liquid and with a calibrated micropipette transfer 35μl of swab sample transport media into a vial of Enhanced Buffer. For each sample to be tested, use an individual vial of Enhanced Buffer.
4. ClosethecapoftheEnhancedBuffervialandmixthedilutedsampleby vortexing for 10 secs.
8.1.3 Lysis
Note: Positive Controls do not require heat-treatment.
IFU-024 Rev 03 Page 11 of 31