Page 5 - Mesenchymal Stem Cell-Derived Exosomes as an Emerging Paradigm for Regenerative Therapy and Nano-Medicine

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comprehensively describe all the facets of analysis of extracellular vesicles. While there are
shortcomings in every technique when used individually, they may be counterweighed by
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the benefits of another technique if explored in a synergistic manner [59,60].

Figure 2. Isolation of Exosomes: exosomes are commonly isolated from the conditioned media. Some common
Figure 2. Isolation of Exosomes: exosomes are commonly isolated from the conditioned media. Some common preprocessing
preprocessing steps are required for both the techniques, including collecting conditioned media from MSCs, performing
steps are required for both the techniques, including collecting conditioned media from MSCs, performing a centrifugation
a centrifugation round at 2000× g for 30 min to remove debris. Furthermore, the conditioned media can be subject to any
round at 2000× g for 30 min to remove debris. Furthermore, the conditioned media can be subject to any of
of the two techniques including, Ultracentrifugation (1) or Kit‐based methods (2) for isolation of exosomes. These the two
exosomes can be further used for characterization, aliquoting, and storage for future experiments.
techniques including, Ultracentrifugation (1) or Kit-based methods (2) for isolation of exosomes. These exosomes can be
further used for characterization, aliquoting, and storage for future experiments.