Page 10 - 毕业论文

The results showed that almost every sample tested was contaminated with
aflatoxins and/or zearalenone to some extent. While ZEA was found in only one
sample, AFG2 was widely spread. According to Medicinal plants &preprations green
trade standards of foreign trade and economic cooperation, none of samples exceeded
the 5 ppb action limit for AFB1, but 17 batches of samples exceeded the 10 ppb
action limit for AF(B1+B2+G1+G2). In respect of origin, the contamination of
mycotoxins in herbal medicines from Guangdong Province was most serious, Hubei
and Guangxi were less and Hunan and Zhejiang were the least. Furthermore, the data
obtained reveals herbal medicines from Radix and Rhizoma were most seriously
contamination by mycotoxins.

3.2 Process optimization for preparation of granules
（1） Preparation of standard decoction

Rhizoma Cimicifugae: 100 g crude drug was cooked three times with 6, 4, 4 volume
(V/M) water, respectively, for 30 min. Before the first cooking, the drug was soaked
in water for 30min.

Radix Sanguisorbae: 100 g crude drug was cooked three times with 6, 4, 4 volume
(V/M) water, respectively, for 30 min. Before the first cooking, the drug was soaked

in water for 30min.
Carthamus tinctorious 100 g crude drug was cooked three times with 10, 8, 8

volume (V/M) water, respectively, for 30 min. Before the first cooking, the drug was
soaked in water for 30min.

（2）Optimal preparation process of granules
Rhizoma Cimicifugae: 100 g crude drug was cooked three times with 10, 8, 8
volume (V/M) water, respectively, for 60 min. The optimized concentration

temperature was set at 70℃.
Radix Sanguisorbae: 100 g crude drug was cooked three times with 10, 8, 8 volume

(V/M) water, respectively, for 45 min. The optimized concentration temperature was
set at 60℃.

Carthamus tinctorius: 100 g crude drug was cooked three times with 10, 8, 8
volume (V/M) water, respectively, for 30 min. The optimized concentration

temperature was set at 80℃.
4 Conclusion
4.1 Analytical method development for simultanteous determination of AFB1, AFB2,

AFG1, AFG2 and ZEA in Chinese herbal medicines using LC-MS/MS
A simple, fast and sensitive method for analysis of AFB1, AFB2, AFG1, AFG2 and

ZEA in Chinese herbal medicines were developed, which may be helpful to establish
a standard method to control the contamination of mycotoxins in Chinese herbal

VI