Page 8 - 毕业论文

Development of an analytical method for determination of

mycotoxins in Chinese herbal medicines using liquid

chromatography/mass spectrometry and process optimization for

preparation of Chinese herbal medicine granules

Speciality: pharmaceutical analysis
Author:Tan Jing

Supervisor: Professor Xu Hui.

Abstract

1 Objective

1.1 To establish a method for simultanteous determination of aflatoxins B1, B2, G1
and G2 (AFB1, AFB2, AFG1 and AFG2) and zearalenone (ZEA) in Chinese

herbal medicines using liquid chromatography/tandem mass spectrometry
(LC-MS/MS).

1.2 To optimize the extraction and concentration process for the preparation of
granules of Rhizoma Cimicifugae, Radix Sanguisorbae, Carthamus tinctorious.

2 Methods
2.1 Analytical method development for simultaneous determination of AFB1, AFB2,
AFG1, AFG2 and ZEA

To develop a suitable method, investigations into the extraction method,
purification method, HPLC conditions (mobile phase, elution gradient and column

used) as well as mass conditions (ion source, parent ions, selection of product ions,
collision energy) were carried out. The established method was further validated

systematically. 75 batches of samples of Chinese herbal medicines obtained from five
different provinces (HuBei, HuNan, GuangDong, GuangXi, ZheJiang) were

determined.
2.2 Process optimization for preparation of granules
Firstly, the standard decoctions of three Chinese herbal medicines studied were

IV