Page 28 - SRL Diagnostics

TECHNOLOGIES & PLATFORMS-CYTOGENETICS

Page 12 Karyotyping
Karyotyping is a detailed analysis of
chromosomes to detect abnormalities of the
number or structure of chromosomes. The
chromosomes are obtained after culture for
72 hours. The metaphases are fixed on
slides, Giemsa banded (G-band), and
analyzed for abnormalities like trisomies,
deletions and translocations.

Coverage: All chromosomes
Reported: 10-14 days
Stage of cell cycle: Metaphase nuclei Page 22 Page 26
Advantage: Visualize all chromosomes

Fluorescence in situ hybridization (FISH)
Fluorescence in situ hybridization (FISH)
is a rapid procedure to detect specific
chromosome rearrangements. This
technique utilizes commercially available
probes complementary to the region of
interest on a particular chromosome. The
analysis is done by counting signals
under the fluorescent microscope as.

Coverage: Target specific sequences
Reported: 3-5 days
Stage of cell cycle: Metaphase &
Interphase nuclei
Advantage: Submicroscopic deletions
not detectable by Karyotyping

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