Page 32 - E-MODUL PEMERIKSAAN RUJUKAN PRODIA

Antiphospholipid Antibody Panel

14890
Test Components:
7352 Cardiolipin Antibodies (IgA, IgG, IgM)
10062 Phosphatidylserine Antibodies (IgG, IgA, IgM)
CPT Code(s): 86146 (x3), 86147 (x3), 86148 (x3)
Includes
Beta-2-Glycoprotein I Antibodies (IgG, IgA, IgM)
Phosphatidylserine Antibodies (IgG, IgA, IgM)
Cardiolipin Antibodies (IgA, IgG, IgM)

Preferred Specimen(s) : 5 mL plasma collected in a 3.2% sodium citrate
(light blue-top) tube
Minimum Volume : 3 mL
Alternate Specimen(s) : Serum
Instructions
Plasma (preferred): Centrifuge light blue-top tube 15 minutes at
approximately 1500 x g within 60 minutes of collection. Using a plastic
pipette, remove plasma, taking care to avoid the WBC/platelet buffy layer
and place into a plastic vial. Centrifuge a second time and transfer
platelet-poor plasma into a new plastic vial. Plasma must be free of
platelets (<10,000/mcL).

Serum: Centrifuge red-top tube 15 minutes at approximately 1500 x g
within 24 hours of collection. Transfer serum into a plastic specimen vial.
Room temperature
Specimen Stability : Room temperature: 4 days , Refrigerated: 7 days
Frozen: 28 days . Reject Criteria : Gross hemolysis • Grossly lipemic

Methodology : Immunoassay (IA)

Clinical Significance
The Antiphospholipid Antibody Syndrome (APS) is a clinical-pathologic
correlation that includes a clinical event (e.g., thrombosis, pregnancy loss,
thrombocytopenia) and persistent positive Antiphospholipid Antibodies
(IgM or IgG ACA >40 MPL/GPL, IgM or IgG anti-B2GPI antibodies, or a
Lupus Anticoagulant). The IgA isotype has been implicated in smaller
studies, but has not yet been incorporated into the APS criteria.
International consensus guidelines suggest waiting at least 12 weeks
before retesting to confirm antibody persistence. Reference J Thromb
Haemost 2006: 4; 295.
(The CPT codes provided are based on AMA guidelines and are for
informational purposes only. CPT coding is the sole responsibility of the
billing party. Please direct any questions regarding coding to the payer
being billed.)

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