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CEN-F-like pattern (AC-14)

HEp-2 cells, autoantibodies against CENP-F show a fine to

coarse speckled fluorescence of the cell nuclei.

The staining intensity varies strongly, G2-phase nuclei

show the strongest fluorescence while G1-phase nuclei

react with much weaker intensity or not at all. Apart from

this, the mitotic cells fluoresce especially strongly

(with the exception of the chromosome region), smooth to

fine speckled.

The centromeres are exclusively positive in the

prometaphase and metaphase and then show many small

and mat aligned dots. In prometaphase cells,the nuclear

membrane is often slightly stained. During ana and

telophase, sometimes an intensive fl uorescence of the

midbody occurs. The cytoplasm of mitotic cells is diffusely

stained.

Primate liver does not show any specifi c reaction