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Chapter 9: Muscle and Nerve Biopsy  95

























               Figure 9.4  With a solid surface under the tibial nerve, a scalpel blade is used   Figure 9.7  An incision has been made in the skin and fascia revealing the
               to split the nerve into one‐third and two‐third sections in a proximal to   underlying common peroneal nerve (arrow). The sites for biopsy of the
               distal direction.                                  proximal biceps femoris and distal gastrocnemius are marked with stars.

























               Figure 9.5  The nerve has been split with a scalpel blade and the proximal
               end is held and transected with a scalpel blade.











                                                                  Figure  9.8  The medial aspect of the forelimb over the elbow has been
                                                                  clipped. An assistant is holding the opposite leg out of the field. A linear
                                                                  incision centered caudally has been made in a proximal to distal direction.
                                                                  The olecranon is marked with an arrowhead and the ulnar nerve bundle
                                                                  with an arrow.


                                                                  The preparation of the tongue depressor is done to ensure it will
                                                                  fit into the planned sample container. In this case a 10‐mL red‐top
                                                                  tube is  usually  chosen. The  subcutaneous tissue  and  skin are
                                                                  closed in routine fashion.
               Figure 9.6  Once the proximal aspect has been severed, the biopsy sample   Sampling of the common peroneal nerve and biceps femoris and
               can be cut at the distal end and prepared as described in the text.  gastrocnemius muscles requires a proximal to distal curvilinear
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