Page 127 - Live-cellanalysis handbook

Kinetic Neuroimmune Assays

Conclusions

These results exemplify the IncuCyte Live-Cell Analysis system’s • Image-based acquisition enables qualitative assessment of
capability for analysis of neuroimmune fucntion in live-cell morphological changes in different microglia in vitro models.
models. Using non-perturbing reagents, a proprietary microplate,
and purpose-built software analysis, microglia can be kinetically • Kinetic measurements of microglia chemotaxis and
evaluated to monitor cell morphology, movement and function phagocytosis capture a complete account of dynamic cell
in a single, flexible platform. Specifically, this system allows for function and movement.
image-based evaluation of changes in microglia morphology
during cell activation and reprogramming events as well as the • All data and time points can be verified by inspecting
kinetic quantification of microglia chemotaxis and phagocytosis individual images and/or time-lapse movies.
in response to physiologically relevant material. The IncuCyte
approach for real-time, long-term quantitative analysis of • Low well-to-well variability and minimal user effort make the
neuroimmune modulation is suited for development and assay amenable to medium throughput screening in a 96- or
characterization of therapeutic approaches for neurodegenerative 384-well format.
disorders.
• This approach also provides a sensitive method to detect
• System allows for users to optimize culture conditions experimental manipulations that alter neuroimmune function
and maintenance of different in vitro models by providing to study microglia function in maintaining brain homeostasis.
automated analysis of cell morphology, movement and
function.

IncuCyte User Publications

1. Balaban S, et al. Adipocyte lipolysis links obesity to breast cancer
growth: adipocyte-derived fatty acids drive breast cancer cell
proliferation and migration. Cancer Metab, 2017, Jan 5:1.
2. Bohlen C. J. et al. 2017. Diverse requirements for microglial survival,
specification, and function revealed by defined-medium cultures.
Neuron; 94 (4): 759-773.e8.
3. Brosius Lutz A. et al. 2017. Schwann cells use TAM receptor-
mediated phagocytosis in addition to autophagy to clear myelin in
a mouse model of nerve injury. Proc. Natl. Acad. Sci. USA; 114 (38):
E8072-E8080.

4. Sellgren C. M. et al. 2017. Increased microglial synapse elimination
in patient-specific models of schizophrenia. https://www.biorxiv.org/
content/early/2017/12/08/231290 Preprint bioRxiv, 231290.
5. Zorina Y. et al. 2018. Human IgM antibody rHIgM22 promotes
phagocytic clearance of myelin debris by microglia. Sci. Rep.; 8:
9392.

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