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Fine-Needle Sampling for Cytologic Analysis: Subcutaneous Masses 1113.e3

or the physical technique was good but the
needle missed the main deposit of diagnostic
VetBooks.ir • When preparing smears for submission
cells). Repeating a nondiagnostic FNA may
be useful.
to a reference laboratory, note that most
laboratories use automated strainers. These
strainers typically do not stain the “distal”
end (i.e., opposite the frosted end) of the
glass slide. As such, it is optimal to keep the
smeared material within the middle half to
two-thirds of the glass slide.
SUGGESTED READING
Boes KM: Fine-needle biopsy for cytology. Clinicians
Brief May:27-31, 2017. https://www.cliniciansbrief. Procedures and Techniques
com/article/fine-needle-biopsy-cytology/.

ADDITIONAL SUGGESTED
READINGS
Meinkoth JH, et al: Sample collection and prepara-
FINE-NEEDLE SAMPLING FOR CYTOLOGIC ANALYSIS: SUBCUTANEOUS MASS Modified Wright tion. In Valenciano AC, et al, editors: Cowell and
stain. Cytology smear of fine-needle aspirate of SQ mass (60× objective) (same case as unstained smears in Tyler’s Diagnostic cytology and hematology of the
separate figure). dog and cat. St. Louis, 2014, Elsevier, pp 1-19.
Meyer DJ: The acquisition and management of cytol-
ogy specimens. In Raskin RE, et al, editors: Canine
and feline cytology: a color atlas and interpretation
of one of the Diff-Quik–stained slides to guide, ed 3, St. Louis, 2016, Elsevier, pp 1-15.
see if there was a reasonable cellular yield.
Unstained smears can still be evaluated AUTHORS: Ryan M. Dickinson, DVM, DACVP; Leah A.
microscopically with the condenser lowered Cohn, DVM, PhD, DACVIM
(if your microscope has this capacity) to allow EDITORS: Leah A. Cohn, DVM, PhD, DACVIM; Mark S.
visualization of the outlines of cell mem- Thompson, DVM, DABVP
FINE-NEEDLE SAMPLING FOR CYTOLOGIC branes and nuclei, which helps determine if
ANALYSIS: SUBCUTANEOUS MASS Unstained there is an adequate yield (see fourth Video).
smear of a fine-needle aspirate of SQ mass. Images • Even with very good technique, cellular yield
from this slide were taken for the previous figure. can be low (lesion may be poorly exfoliative

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