Page 533 - The Veterinary Laboratory and Field Manual 3rd Edition

470 Regula Waeckerlin and Susan C. Cork

Figure 14.2 Ecological considerations for the sampling of ticks. Special considerations in the diagnostics
of arthropod-borne viruses – virology.

cell culture A wide range of mammalian cells can also be
used for arbovirus detection, most commonly
In the case of arthropod-borne viruses, virus kidney cells such as Vero (monkey), BHK-21
isolation in insect cells, tick cells or mamma- (hamster) or LLC-MK2 (pig) cells are used.
lian cells is an option. There are over 40 tick While tick-borne viruses rarely replicate in
cell lines available, which were all derived from insect cell lines, insect-borne viruses replicate
hard ticks (Ixodidae). Most commonly used for in tick cells quite readily, although sometimes
arbovirus detection are IDE2 cells, derived from requiring several passages. Both insect- and
Ixodes scapularis. Insect cell lines are equally tick-borne arboviruses usually replicate quite
readily available. By far the most popularly used readily in mammalian cells. Tick-borne viruses
insect cell line for the detection of arboviruses is tend to cause cytopathic effects in mammalian
the C6/36 cell line derived from Aedes albopictus. cell lines, but persistent infections in tick cells,

Vet Lab.indb 470 26/03/2019 10:26