Page 617 - The Veterinary Laboratory and Field Manual 3rd Edition
P. 617
554 Index
multiple resistance 232–3 molecular application as diagnostic tool 262–3
reading/interpreting results 229–30 DNA restriction 268–73
selecting antimicrobial drug 233–4 hybridization 263–4
biochemical tests 218 probes/microarrays 263–4, 268
beta-galactosidase 218 sequencing 273–6
catalase 218 techniques 263–4, 268–76
citrate 219 morphology/classification 202–3
coagulase 219 mycology 234
gelatine liquefaction 219 appearance of skin scales, nails, crusts in KOH
hydrogen sulphide production 219 preparation 238
methyl red (MR) reaction 219 choice of media 238–9
motility 220 culture 238
nitrate reduction 219–20 direct microscopy 238
oxidase (cytochrome oxidase) 220 fungal infections of the skin 237
oxidation fermentation 218 fungi commonly isolated from cases of
urea hydrolysis 220 ringworm/other skin conditions 239
Voges-Proskauer (VP) reaction 219 laboratory diagnosis 237
cell components/function moulds/yeast 234–6
capsule 201 mycotoxins 240
cell wall 201 other fungal pathogens 239
cytoplasm 201 specimens to collect 237
cytoplasmic membrane 201 necropsy samples 366
endospores 202 sample collection, preparation, handling 198–9
fimbriae/pill 202 sample collection, preparation, submission 203
flagella 201 laboratory examination of specimens 204–5
genetic information 201 staining characteristics 205–6
commercial kit tests sequencing 273
domestic carnivores 261 MALDI-TOF 275–6
equine 256–7 multi-locus sequence typing (MLST) 273–4
pigs 257–9 next-generation sequencing/metagenomics 275
poultry 259–61 serological methods 249–50
ruminants 254–6 agar gel immunodiffusion test (AGID) 250
culture/identification of bacteria 208 agglutination/precipitation 250
biochemical tests 218–20 benefits/limitations of rapid ’pen side’ tests
characteristics to record 214–17 253, 261
commercial systems 220–4 complement fixation 250
goals of primary inoculation 214 CPE inhibition 251
media/special requirements 208–13 ELISA 252
pH requirements 213–14 haemagglutination-inhibition (HI) test 252
quantitative tests/counting bacteria 224–5 neutralization tests 250–1
routine stains used 217–18 prions 253
serological typing 222, 224 virus haemagglutination (HA) test 252
temperature 214 viral culture methods 245
DNA classification of viruses 265–7 cell culture 246–8
DNA restriction 268 virus propagation in embryonated chicken eggs
amplification 268–9 249
binary typing using PCR 271 virology 240
conventional PCR 269 diagnosis of viral infections 241–2
isothermal amplification 271–3 electron microscope 245
real-time PCR (aPCR) 269–71 immunofluorescent microscope 245
media/special requirements isolation/identification of some viruses 243
agar plates 209 light microscope 243–5
anaerobic jar 210 properties of viruses 241
isolation/identification of common bacterial serological methods 249–62
pathogens 210 viral culture methods 245–9
media 211 mineral/trace element assays 351
miniaturized biochemical test kits 223 minerals 339
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