Page 67 - The Veterinary Laboratory and Field Manual 3rd Edition

36 Susan C. Cork, Roy Halliwell and Willy Schauwers

Table 1.3 Suggested materials for a simple sample collection kit.

Item Number Comments
Cool box, 2 cool packs (foam type 1
preferred) or dry ice
Pen, pencil, pencil sharpener, marker, 1 Fill in the disease report form and laboratory
note book and/or data sheets submission form!
Scalpel and scalpel blades size 22 10 Always bring a small plastic container to put used
needles and scalpel blades in – do not leave them
behind as someone may injure themself.
Syringes and needles (20–26 Gauge), 10 More vacutainers will be required if it is planned
7 ml vacutainers EDTA and plain, to collect serum samples for disease screening.
vacutainer holder
Cryovials 5 ml 10
Cryovials with 1 ml brain heart infusion 5
transport medium for avian influenza and
Newcastle disease swabs
Universal bottle with 10 ml PBS 5
Pasteur pipettes, plastic 10
Transport medium for bacteriology 5
Cotton wool and/or swabs 1
Slides (one frosted end), fixative and 50 slides
slide box for blood smears 1 slide
box
Ethanol 200 ml Use ethanol for fixing blood smears.
Coplin jar (50 ml) 1
Tooth picks 1 pack For simple slide agglutination tests.
70% alcohol and 10% buffered formalin 500 ml Use for small tissue sections for histology/
in a well-sealed plastic jar biopsy material and/or preservation of parasite
specimen.*
Small plastic containers, sterilin type 10 Use for small specimens.
(20 ml)
Sealable plastic bags. 30 Use for collection of faecal samples.
Wide mouth bottles with screw cap 2 Use for PM sampling.
Small torch, scissors and tweezers and 1 Make up your own kit of ‘useful’ items which
any specific diagnostic reagents required may be required.
for simple rapid field tests**
Swabs with and without transport 20 of Collection of microbiology samples may not be
medium each very productive unless there is the facility to keep
them cool.
Disposable surgical gloves 1 pack Make sure that there is protective clothing for
Plastic aprons. 2 sample collection and /or PM work.
Heavy duty rubber gloves 2
Disinfectant Small Make sure that staff is advised about the risk of
bottle or zoonotic diseases.
sachets

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