Page 305 - Natural Antioxidants, Applications in Foods of Animal Origin
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284                Natural Antioxidants: Applications in Foods of Animal Origin
  VetBooks.ir  Gad and Abd El-Salam (2010) monitored the effect of addition of different


            concentration of rosemary extract (RE) to skim milk during processing for
            production of soft cheese. The antioxidant activity of the blends of skim milk
            and REs was improved by heat treatment. The addition of calcium chloride
            and pasteurization further significantly increased the phenol content and the
            antioxidant activity of skim milk, whereas addition of sodium chloride and
            homogenization of the skim milk-REs did not affect antioxidant activity.
            Skim milk with high rosemary concentration was reported to have high
            antioxidant activity.
               In a subsequent study, ultra-filtered (UF)-soft cheese was prepared from
            UF milk retentate (1.5% fat) and supplemented with 1–5% RE and cold
            stored for 30 days (El-Din et al., 2010). The TPC and antioxidant capacity
            were evaluated using DPPH and FRAP methods in retentate before and after
            pasteurization and salting along with the resultant cheese. Fortification of
            retentate with RE increased its phenolic content and consequently, its anti-
            oxidant activity. Pasteurization increased the TPC and antioxidant activity.
            It was observed that addition of 3% NaCl reduced slightly the radical scav-
            enging activity (RSA), TPC, and FRAP values. Moreover, it was noticed
            that UF-soft cheese fortified with 1% RE retained more TPC and antioxidant
            activity; also, increasing the concentration of RE to 5% had more acceptable
            flavor, body and texture, and antioxidant activity until 30 days. Furthermore,
            the rate of decrease in TPC, RSA (%), and FRAP values in cheese samples
            containing RE after 30 days of storage were less as compared to control
            cheese (without RE) (El-Din et al., 2010).
               A functional  cheese  product containing  polyphenolic  compounds
            was  developed,  and  the  polyphenolic  retention  efficiency  and  antioxi-
            dant property of the product was evaluated  by Han et al. (2011). Single
            phenolic compounds, including catechin, epigallocatechin gallate (EGCG),
            tannic acid, homovanillic acid, hesperetin and flavones, and natural crude
            compounds, such as grape extract, green tea extract, and dehydrated cran-
            berry powder, were added as functional ingredients to the prepared cheese.
            Cheese curds with polyphenolic compounds at a concentration of 0.5 mg/
            mL showed effective  free  radical-scavenging  activity  and showed high
            retention coefficient ranging between 0.74 and 0.87. The nutritional value
            of cheese product was reported to be improved by adding bioactive phenolic
            compounds to the cheese curd.
               Olmedo et al. (2013) evaluated the preservative effect of oregano and
            rosemary  essential  oils  on  the  oxidative  and  fermentative  stabilities  of
            flavored cheese prepared with cream cheese base. The addition of oregano
            and  rosemary  essential  oils  was reported  to  improve  the  oxidative  and
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