Page 91 - Live-cellanalysis handbook

Kinetic Multi-Spheroid Assays

Conclusions

The use of multi-spheroid assays as model systems for the • Link morphology, growth, and toxicity via direct, kinetic
evaluation of new treatments and study of disease has expanded measurements by combining novel DF Brightfield image
in recent years. However, despite their ability to measure acquisition and analysis with IncuCyte® Cell Health Reagents.
indicators of cell health, such as cytotoxicity and apoptosis, • Investigate mechanisms of action with multiplexed viability and
common assays rely on indirect, end-point measurements toxicity measurements using non-perturbing reagents and cell
that are subject to artefacts and cannot be readily verified lines that stably express fluorescent proteins.
by morphological changes. The IncuCyte S3 Live-Cell Analysis
System’s Multi-Spheroid assay is a rapid method for the • Produce reproducible well-to-well kinetic data in live spheroid
pharmacological investigation of potential drugs in a meaningful cultures, in response to different compounds and conditions,
multicellular model, which allows researchers to: using lab-tested protocols, high quality images, and unbiased
analysis.
• Study spheroid morphology, growth and shrinkage using a • Carry out automated analysis of multiple conditions or variables
label-free, scaffold-based, kinetic 96-well plate assay without under more physiologically relevant conditions, minimizing
removing cultures from the incubator and without need for a interference with cell biology.
predefined endpoint selection.

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