Page 50 - IBRO_RNA School_Abstract Book
P. 50
Answer: Malat1
(b)Is this gene a coding gene or a non-coding gene?
☐Coding gene
☐Non-coding gene
It is a non -coding gene; you can see this from the thickness of the blue line. Coding
regions are shown with a thick bar.
(c)If you use this primer pair for RT-qPCR, what is the expected product size (in basepairs)?
Answer: 123bp (slight differences may be expected if you are using click and drag to
zoom in. Some people answered by giving the range of sizes acceptable in a RT-PCR
reaction. The question was about using the browser to find the exact, expected size from
the genome.
Question-3
The following primers can be used to detect the expression of which gene in mouse?
Forward primer- CTGATCGTTTGGTGCTGTGT
Reverse primer- CCCTGAACACCCACTCAGTT
☐Specifically Xist
☐Specifically Tsix
☐Both Tsix and Xist
☐Neither Tsix nor Xist
Ans: Specifically XIST (see below; pay attention to the orientation of forward and reverse
primers)
