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International Conference on

                                  Recent Trends in Environmental Sustainability


                                                    ESCON22/FWSH/19
               Screening for fast-growing oleaginous microalgae as potential source of algal oils

                                                                                                 3
                                                                                  1
                                                             1
                                         1,2
               Abdullahi Balarabe Inuwa , Qaisar Mahmood , Muhammad Irshad , Farman Ullah ,
                                         1*
                           4
               Iftikhar Zeb , Rashid Nazir
               1 Department  of  Environmental  Sciences,  COMSATS  University  Islamabad  (CUI)  –
               Abbottabad Campus.
               2 Department of Microbiology, Faculty of Life Sciences, College of Natural and Pharmaceutical
               Sciences, Bayero University Kano, Nigeria.
               3 Life  Sciences  Center  (LiSCENT),  COMSATS  University  Islamabad  (CUI)  –  Abbottabad
               Campus.
               4 Department  of  Biotechnology,  COMSATS  University  Islamabad  (CUI)  –  Abbottabad
               Campus.

               Correspondence: rashidnazir@cuiatd.edu.pk
               Abstract

               Microalgal oils receive a great deal of attention as potential future source of  biodiesel and
               organic  dietary  supplements.  However,  large-scale  production  of  these  oils  requires  the
               preliminary careful selection of superior microalgae feed stocks.  Here, we report the screening
               of microalgae culture collection in search of rapid growing, oil-rich strains. The aim was to
               choose strains that would foster high yield of lipids under large-scale cultivation conditions.
               To  that  end,  batch  cultivation  of  57  strains  was  carried  out  on  a  BG11  medium  under
               photoautotrophic conditions.  Growth rate of the strains was monitored at 2-day interval and
               total lipid content was determined at the end of cultivation.  Based on the results obtained, a
               screening pipeline was established and a total 13 strains were selected accordingly. The growth
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               rate ranged from 0.0867 ± 0.002 d-  to 0.139 ± 0.005 while the lipid contents ranged from
               28.17 ± 0.133 to 43.647% ± 0.26. Strains AY4, CM2, CM6, KL10 and LY2 were found to fit
               the bill most as they combine together both the high lipid contents and rapid growth rate. The
               lipid contents compared favorably with many reports from previous literatures, while growth
               rate  was  moderate  and  reflected  the  sub-optimal  cultivation  conditions  used.    It  can  be
               concluded  that  the  screening  process  yielded  a  suite  of  microalgae  strains  that  could  be
               potentially utilized as sustainable source of microalgae oils.
               Keywords: microalgae; oleaginous, screening, lipid content, growth rate

























                 Department of Environmental Sciences, COMSATS University Islamabad, Vehari Campus

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