Page 69 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

G Ge en ne er ra al l p pr ri in nc ci ip pl le es s

Introduction:
 The term "chromatography is derived from the Greek words chromos and graphy,

meaning "color" and "writing", respectively.
 Chromatography is the art of separation technique for the identification, and

determination of individual chemical components in complex mixtures.
 It is a physical method of separation, in which the components to be separated are

distributed between two phases, one of these phases constituting a stationary bed of

large surface area "stationary phase", the other being a fluid "mobile phase" that

percolates through or along the stationary bed.

 Stationary phase is that which does not move during the separation process.

 Mobile phase "eluent" is a fluid used to carry the components of a mixture

through a stationary phase.

 Elution: is process by which solutes are washed through a stationary phase by the

movement of a mobile phase.

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