Page 72 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

6) Cover the beaker with a watch glass.
7) Leave the beaker until the mobile phase becomes about 2-cm from the top of the filter

paper, remove the paper from the beaker, make a mark at the solvent front and leave
the paper to dry.

N.B.:

The mobile phase separated the dye into 2 spots (blue and yellow spots).

Calculate Rf values for each of the two separated components.

The distance* travelled by the substance
R f =
The distance traveled by the solvent

* the distance is measured from the center of the spot.

44 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5