Page 76 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

P Pr ro oc ce ed du ur re es s: :

1 1- - P Pr re ep pa ar re e t th he e d de ev ve el lo op pi in ng g c co on nt ta ai in ne er r

1- The developing container for TLC can be a specially designed chamber, a jar with a lid,

or a beaker with a watch glass on the top.

2- In the teaching labs, we use a beaker with a watch glass on

top.

3- Pour solvent into the beaker to a depth of

just less than 0.5 cm.

2 2- -P Pr re ep pa ar re e t th he e T TL LC C p pl la at te e

1- Measure 0.5 cm from the bottom of the plate.

2- Take care not to press so hard with the pencil that you

disturb the adsorbent.

3- Under the line, mark lightly the name of

the samples you will spot on the plate

3 3- -S Sp po ot t t th he e T TL LC C p pl la at te e

Do this process 3 times

4 4- -D De ev ve el lo op p t th he e p pl la at te e

1- Place the prepared TLC plate in the developing beaker, cover the beaker with the

watch glass, and leave it undisturbed on your bench top. Run until the solvent is about

half a centimeter below the top of the plate.

2- Remove the plate from the beaker and quickly mark a line across the plate at the

solvent front with a pencil.

48 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5