Page 77 - Practical book PC406-MNU-2025
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MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)
3- Allow the solvent to evaporate completely from the plate.
4- If the spots are colored, simply mark them with a pencil. Most samples are not
colored and need to be visualized with a UV lamp. Hold a UV lamp over the plate and
mark any spots which you see lightly with a pencil.
R RE ES SU UL LT TS S
1. Provide sketches of the TLC plates. Label each spot on the
sample plate by identifying the substance as AC, AS or IB.
2. Based on your experimental results, list the substances found in
the analgesic drug mixture you analyzed. Record the Rf values
calculated for each substance found in the mixture.
49 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5
