Page 78 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

II- Column Chromatography

DEFINITION OF TERMS RELATED TO THE CHROMATOGRAM

TERM DEFINITION
 That portion of the chromatogram recorded when only the

Base Line
mobile phase emerges from the column.

 That portion of the chromatogram recorded while a single

component emerges from the column (or, if separation of a
Peak
mixture has been in incomplete, two or more unseparated

components may appear as one peak).

 An interpolation of the base line between the extremities of
Peak Base
the peak.

Peak Area  The area enclosed by the peak and the peak base.
 The distance from the peak maximum to the peak base.
Peak Height
 The magnitude of the peak base intercepted by the tangents
Peak Width (W):
to the inflection points of the peak.

 The time between sample injection and the appearance of a
Retention time
(tR): solute peak at the detector of a chromatographic column.

 The time it takes for an unretained species (mobile phase) to

Dead time (tM):
pass through a column.
adjusted  the time solute spent in the stationary phase, and equals to
Retention Time [tR -tM].
'
(tR)

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