Page 82 - Practical book PC406-MNU-2025
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MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)
Interpretation of Chromatograms
The data acquired from the chromatogram:
tm , tRA , tRB , WA , WB , ............. cm
The required chromatographic parameters:
Parameters calculated for each drug (each peak)
{i.e. calculated 2 times each time for one drug}
Number of theoretical plates (NTP):
2
NTP = 16 ( tR )
w
Height equivalent to theoretical plates (HETP):
HETP = L
NTP
'
Capacity factor (K ) = tR tM = R t
tM tM
54 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5
