Page 86 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

Another example for Chromatogram

You are provided with a typical chromatogram resulted from separation of a certain
ternary mixture (A, B and C) using C 8 column of a length 20 cm. Use this chromatogram

to obtain values of the following required parameters:

Where; (s) Solvent front.

Considering previous figure complete the following table

Required Parameter Value

1- NTP (A)

2- NTP (C)

3-HETP (A)

4- HETP (B)

5- K' (A)

6- K' (C)

7- Resolution for A and C

8- Selectivity factor for B and C

58 | P a g e S e c o n d s e m e s t e r 2 0 2 4 - 2 0 2 5