Page 70 - Practical book PC406-MNU-2025

MANSOURA NATIONAL UNIVERSIY
PHARM D- CLINICAL PHARMACY LEVEL II INSTRUNENTAL ANALYSIS (PC 406)

I- Planar chromatography

 Planer Chromatography: in which the stationary phase is supported on a flat plate

or in the pores of a paper.

 The mobile phase moves through the stationary phase by capillary action,

sometimes assisted by gravity or an electrical potential.

 Planer chromatography falls into three forms:

 It is a technique in which a sheet or strip of filter

a) paper paper serves as a stationary phase.

chromatography  Usually, special papers, which are highly purified

(PC) and reproducible as to porosity and thickness, are

employed.

b) Thin-layer  It is a technique in which the stationary phase

chromatography consists of a narrow layer of finally divided coated

(TLC) or uncoated particles supported on a glass plate.

c) Electrophoresis and  It is a technique in which the components of the

Electro sample are separated by its migration through a

chromatography suitable phase under the influence of an electrical

(EC) field.

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