Page 114 - phytochemistry general program

Affinity chromatography .3

Affinity chromatography makes use of the affinity of proteins to specific ligands,
such as enzyme substrates or close analogues. The ligands which may have a low
or high molecular weight are coupled to suitable derivatives of cellulose, dextran,
polyacrylamide or agarose or even to glass beads. It is generally used for

purification of proteins and nucleic acids.

Zone electrophoresis .4

Electrophoresis refers to the displacement of charged particles in a fluid under the
influence of an electric field. The stationary phase is usually a support moistened
with a buffer solution. It works by the application of a suitable potential across the

medium. It is used for separating ionized substances such as proteins.

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