Page 105 - Phytochemistry 2 (06-PG 605)

Classification of chromatography

1-Types
According to the physical phenomena involved in distribution of
compounds (solutes) between the two phases
1-Partition chromatography
2-Adsorption chromatography
3-Ion exchange chromatography
4-Size exclusion chromatography
5-Affinity chromatography
6-Electrophoresis

2- Techniques
A- planar

1-Paper chromatography
2-Thin layer chromatography, TLC,
3-Centrifugal chromatography (chromatotron)

https://youtu.be/fOOtUIppdJw
Mobile phase (developing system)

B-column chromatography
Based on the applied pressure:
a)-open column (gravity column, conventional column)
b)-pressure liquid chromatography

1-high pressure liquid chromatography (HPLC, UPLC)
2-flash chromatography
3-medium pressure chromatography

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