Page 110 - Phytochemistry 2 (06-PG 605)

2- Ion exchange chromatography(LSC)

Used for ionic substance in aqueous solution

Stationary phase (ion exchanger):

Matrix (inert polymer) attached to it a fixed ion (charged particles

+ve or –ve charge & a counter ion is loosely attached to the fixed

ion

Matrix fixed ion counter ion

+ve ------------ –ve anion exchanger── Resin

NR+4 OH- or Cl-

–ve ------------ +ve cation exchanger

COO- or SO-3 H+

The counter ion is exchanged by the analyte (solute ion)

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