Page 112 - Phytochemistry 2 (06-PG 605)
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3-Size exclusion (Gel) chromatography (LSC)
Separation depends on molecular size, shape, weight.
It is not used for isomers)
Stationary phase
polymeric substance with certain controlled pore size
Elution is isocratic
Applications:
o Separation, fractionation, purification of polymers (proteins,
polysaccharides ,…..)
o Determine molecular weight of polymers.
Size exclusion (Gel) chromatography
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