Page 115 - Phytochemistry 2 (06-PG 605)
P. 115
5-Partition chromatography (LLC)
partitioning of sample components between two immiscible
(partially miscible) phases.
Partition chromatography can be applied in TLC, paper & column
techniques
o The stationary phase is a liquid held on a suitable inert
support
o The mobile phase is liquid (single solvent or mixture of
solvents)
the elution is isocratic
o The separation occurs as a result of differences in partition
coefficient
o The Temperature must be kept constant during the process
(it affects
equilibrium)
Classification
According to the polarity of st.ph. and m. ph. It is classified to
1-Normal phase
Polar st.ph. & non polar m.ph.
the compounds are eluted in order of increasing polarity
(least polar solute eluted first).
2-Reversed phase
Non polar st.ph. & polar m.ph.
the compounds are eluted in order of decreasing polarity
(most polar solute eluted first).
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