Page 119 - Phytochemistry 2 (06-PG 605)

Centrifugal (Chromatotron)
o Preparative centrifugally accelerated, radial thin layer
o Used mainly for preparative purposes (isolation)
o The rotor is allowed to rotate mechanically at about 1000
rpm.
o The separated compounds appear as concentric zones

Paper chromatography
stationary phase
sheet of whatman filter paper of different grades (thickness). No.1,
No.2 ,………No.30 ,….
Whatman filter paper made of cellulose is hydrophilic, used as
normal phase
Hydrophobic whatman filter paper (acetylation of OH of cellulose is
used as reversed phase
The principle of separation is mainly partition. Like attracts like
More polar compound is more retained on cellulose paper and do
not travel so far

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