Page 116 - Phytochemistry 2 (06-PG 605)
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Planar Techniques Thin layer chromatography ( TLC )
TLC is a sensitive technique.
Can analyze microgram quantities.
It takes little time for an analysis (5-10 minutes)
The sample is dissolved in a volatile solvent to produce a very
Principle:
dilute solution (~1%)
TLC plate
Developing system (m.ph.)
Tank (TLC jar)
U V or spraying reagent (visualizing agent), sprayer (atomizer)
TLC involve Three steps:
spotting, development, and visualization.
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