Page 38 - Phytochemistry 2 (06-PG 605)

( - ) Quinine (+) quinidine

when warmed with KOH in amyl alcohol.

Totaquine:

is a combination of total alkaloids of cinchona and is employed for the same

purpose of quinine.

Identification of cinchona alkaloids

1- Thalleioquin test
Few drops of bromine water + solution of quinine salt, + ammonia solution
emerald green colour is produced.

This reaction is sensitive to quinine (0.005% solution give +ve reaction).

• It is also given by quinidine but not with cinchonidine or cinchonine.

2- Fluoresence test
• Quinine and quinidine show blue fluorescence with dil. oxygentated acids
(e.g. sulphuric or phosphoric )

Estimation of Cinchona alkaloids
 spectrophotometric method
 Gravimetric method

Action and uses of Cinchona alkaloids
• Quinine has antimalarial activity in a dose of 300-600 mg of quinine sulfate

or other salt for 14 days.

• Quinine is used as bitter tonic (tonic water).

• Quinidine is used for treatment of auricular fibrillation in a dose of 200 mg of
quinidine sulfate daily

37