Page 119 - Applied Pharmacognosy

Mobile Phase

❑ Mobile phase must be Liquid and it is placed in the mobile
phase reservoir in the HPLC system.

Aqueous - Polar Organic - Non-polar

❑ Solvents used has to be of HPLC grade; regular solvents
cannot be used.

Characteristics of HPLC solvents

❑ Purity. 118
❑ Low viscosity.
❑ Solubility of the sample.
❑ Chemical inertness.
❑ Detector compatibility.
❑ Reasonable price.