Page 124 - Applied Pharmacognosy
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Mobile Phase Handling

                          Off-line Degassing

❑ Helium degassing = Purging or sparging or Gently bubbling
   He through the mobile phase for a few minutes.

❑ Ultrasonication = Sonic baths to mix & degas mobile phases.
❑ Vacuum degassing = Applying a vacuum alone or in

   combination with filtration.

                                                       Fluoropolymer Filter-Degasser

Helium Degasser                                                                     123

                 Vacuum Degassing : filtration + degassing
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