Page 128 - Applied Pharmacognosy

Hydrophobic Interactions

Reversed Phase

H2O H2O If nonpolar solute H2O H2O H2O
is added...
H2O Nonpolar solute H2O
H2O
H2O H2O H2O
H2O H2O H2O

Network of hydrogen bonds …the network is broken and...

H2O H2O H2O
H2O
H2O H2O H2O …the nonpolar substance
is pushed to a nonpolar
Nonpolar solute location.

Nonpolar stationary phase 127