Page 130 - Applied Pharmacognosy
P. 130
Effect of Chain Length of Stationary Phase
Reversed Phase
❑ In general, longer chains have a greater retention strength.
C18 (ODS) C8
Strong Medium
❑ Reversed-phase chromatography C4
eliminates peak tailing because the Weak
stationary phase has few sites that
can strongly adsorb a solute to cause 129
tailing.
