Page 135 - Applied Pharmacognosy

Mobile Phase Selection

How to adjust the Mobile phase Strength?

❑ Mobile Phase Strength is 60/40
adjusted by changing: 70/30
➢ Type of Modifier (MeOH,
ACN).
➢ Solvent Strength (%
modifier), has a greater
influence on separation.

Eluent: Methanol / Water

As polarity of eluent 80/20
Overall retention time
134
Reversed Phase Separation